| Peer-Reviewed

Nucleic Acids in Atypical Conditions

Received: 26 March 2015     Accepted: 17 April 2015     Published: 29 April 2015
Views:       Downloads:
Abstract

Here we describe some properties of nucleic acids, which observed in ‘atypical” experiments. So, we show that physical and chemical properties of nucleic acids depend on dissolved gases. We show particularly that thermal hyperchromism of DNA depends on dissolved oxygen and (or) air. It has been showed most importantly the thermal hyperchromism does not observe for DNA degassed solutions. Here we also demonstrate the difference between some chemical properties of nucleic acids in degassed solutions and in solutions saturated with hydrogen or oxygen. We illustrate too the difference between physical and chemical properties of nucleic acids in the homogenous and gradient-containing solutions; the last case we offer as approximation of the conditions, in which the nucleic acids exist in vivo.

Published in European Journal of Biophysics (Volume 3, Issue 1)
DOI 10.11648/j.ejb.20150301.12
Page(s) 5-9
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

DNA, RNA, DNA Hyperchromism, DNA Melting, Phenazine

References
[1] Saenger, W. (1993) Principles of nucleic acid structure. New York Berlin Heidelberg Tokyo: Springer-Verlag.
[2] Doshi, R., Day, P. J. R., and Tirelli, N. (2009) Dissolved oxygen alteration of the spectrophotometric analysis and quantification of nucleic acid solutions. Biochemical Society Transactions, 37(2), 466–470.
[3] Doshi, R., Day, P.J.R., Carampin, P., Blanch, E., Statford, I.J. and Tirelli, N. (2010) Spectrophotometric analysis of nucleic acids: oxygenation-dependant hyperchromism of DNA. Anal. Bioanal. Chem., 396, 2331–2339.
[4] Le Pecq, J.-B., Le Bret, M., Barbet, J. and Roques B. (1975) DNA polyintercalating drugs: DNA binding of diacridine derivates. Proc. National Academy Science USA. 72( 8), 2915–2919.
[5] Tanious, F.A., Yen, Sh.-F. and Wilson, W.D. (1991) Kinetic and Equilibrium Analysis of a Threading Intercalation Mode: DNA Sequence ad Ion Effects. Biochemistry 30(7), 1813–1819.
[6] Yen, S.-F., Gabbay, E.J. and Wilson W.D. (1982) Interaction of Aromatic Imides with Deoxyribonucleic Acid. Spectrophotometric and Viscometric Studies. Biochemistry. 21(9), 2070–2076.
[7] Wilson, W.D., Tanious, F.A., Barton, H.J., Jones, R.L., Fox, K., Wydra, R.L. and Strekowxki, L. (1990) DNA Sequence Dependent Binding Mades of 4',6-Diamidino-2-phenylindol (DAPI). Biochemistry. 29(36),.8452–8461.
[8] Kastrup, R.V., Jong, M.A. and Krugh, Th.R. (1978) Ethidium Bromide Complexes with Self-Complementary Deoxyribonucletides. Demonstration and Discussion of Sequence Preferences in the Interactive Binding of Ethidium Bromide. Biochemistry. 17(23), 4855–4865.
[9] Veselkov, A.N., Devis, D., Dymant, L.N. and Parkes H. (1991) Study of the interaction proflavine with deoxytetraribonucleoside triphosphate 5`-d(ApCpGpT) by one- and two- dimensional IН-NMR. Byopolymers and cell. 7(6), 5–15.
[10] Radzevilova, O.P., Nikitina, V.V., Fedotova, O.V. Ratushnaja, E.V. and Borodulin, V.B. (2000) Interaction of thiapirillium salts with DNA. Molecular biology. 34(1) 95–100.
[11] Hollstein, U., and Van Gemert, R.Y.jr. (1971) Interaction of phenazines with polydeoxyribonucleotides. Biochemistry, 10(3), 497–504.
[12] Blagoj, Yu.P., Zozulja, V.N., Voloshin, I.M., Makitruk, V.L., Shalamai, A.S. and Sherbakova, A.S. (1997) Investigation of the interaction of phenazine derivates with DNA by polarized fluorescence. Byopolymers and cell..13(1), 22–29.
[13] Moroshkina, E.E. and Safjannikova, M.G. (1999) Interaction of DNA with phenazine derivates. Biophysics. 44(3), 425–429.
[14] Long, E.C. and Barton, J.K. (1990) On demonstrating DNA interaction. Acc. Chem. Res. 23(9), 273–279.
[15] Harris, G. (1973) Nuclei and cytoplasm. Moscow: Mir.
[16] Biochemie ABC. (1976) Leipzig: VEB F.A. Brockhaus Verlag.
[17] Moore, R.D. and Morrill, G.A. (1976) A possible mechanism for concentrating sodium and potassium in the cell nucleus, Biophys. Journ. 16, 527–533.
[18] Hooper, G. and Dick, D.A.T. (1976) Nonuniform distribution of sodium in the rat hepatocyte. Journ. of General Physiology. 67, 469–474.
[19] Oberleihtner, H., Wunsch, S. and Schneider, S. (1992) Patchy accumulation of apical Na+transporters allows cross talk between extracellular space and cell nucleus. Proc. Nat. Acad. Sci. USA. 89, 241–245.
[20] Marmur, J. and Doty, P. (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol., 5, 109–118.
[21] Mergny, J.-L., Li, J., Lacroix, L., Amrane, S. and Chaires, J. B. (2005) Thermal difference spectra: a specific signature for nucleic acid structures. Nucleic Acids Res., 33(16), 1–6.
[22] Nagai, K.and Hecht, S.M. (1991) Site-specific Cleavage by Antisense Oligonucleotides Covalently Linked to Phenazine Di-N-oxide. J.B.C. 266(35), 23994 – 24002.
[23] Nagai, K., Carter, B.J., Xu, J. and Hecht, S.M. (1991) DNA Cleavage by Oxygen Radicals Produced in the Absence of Metal Ions or Light. J. Am. Chem. Soc. 113, 5099 – 5100.
[24] Pivovarenko, Y.V., Shablykin, O.V. and Vasiljev A.N. (2012) The nature of interactions between cationic phenazine-N-oxide and DNA. Medical chemistry (Ukr. Ed.), 3, 20–24.
Cite This Article
  • APA Style

    Yuri Pivovarenko. (2015). Nucleic Acids in Atypical Conditions. European Journal of Biophysics, 3(1), 5-9. https://doi.org/10.11648/j.ejb.20150301.12

    Copy | Download

    ACS Style

    Yuri Pivovarenko. Nucleic Acids in Atypical Conditions. Eur. J. Biophys. 2015, 3(1), 5-9. doi: 10.11648/j.ejb.20150301.12

    Copy | Download

    AMA Style

    Yuri Pivovarenko. Nucleic Acids in Atypical Conditions. Eur J Biophys. 2015;3(1):5-9. doi: 10.11648/j.ejb.20150301.12

    Copy | Download

  • @article{10.11648/j.ejb.20150301.12,
      author = {Yuri Pivovarenko},
      title = {Nucleic Acids in Atypical Conditions},
      journal = {European Journal of Biophysics},
      volume = {3},
      number = {1},
      pages = {5-9},
      doi = {10.11648/j.ejb.20150301.12},
      url = {https://doi.org/10.11648/j.ejb.20150301.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejb.20150301.12},
      abstract = {Here we describe some properties of nucleic acids, which observed in ‘atypical” experiments. So, we show that physical and chemical properties of nucleic acids depend on dissolved gases. We show particularly that thermal hyperchromism of DNA depends on dissolved oxygen and (or) air. It has been showed most importantly the thermal hyperchromism does not observe for DNA degassed solutions. Here we also demonstrate the difference between some chemical properties of nucleic acids in degassed solutions and in solutions saturated with hydrogen or oxygen. We illustrate too the difference between physical and chemical properties of nucleic acids in the homogenous and gradient-containing solutions; the last case we offer as approximation of the conditions, in which the nucleic acids exist in vivo.},
     year = {2015}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Nucleic Acids in Atypical Conditions
    AU  - Yuri Pivovarenko
    Y1  - 2015/04/29
    PY  - 2015
    N1  - https://doi.org/10.11648/j.ejb.20150301.12
    DO  - 10.11648/j.ejb.20150301.12
    T2  - European Journal of Biophysics
    JF  - European Journal of Biophysics
    JO  - European Journal of Biophysics
    SP  - 5
    EP  - 9
    PB  - Science Publishing Group
    SN  - 2329-1737
    UR  - https://doi.org/10.11648/j.ejb.20150301.12
    AB  - Here we describe some properties of nucleic acids, which observed in ‘atypical” experiments. So, we show that physical and chemical properties of nucleic acids depend on dissolved gases. We show particularly that thermal hyperchromism of DNA depends on dissolved oxygen and (or) air. It has been showed most importantly the thermal hyperchromism does not observe for DNA degassed solutions. Here we also demonstrate the difference between some chemical properties of nucleic acids in degassed solutions and in solutions saturated with hydrogen or oxygen. We illustrate too the difference between physical and chemical properties of nucleic acids in the homogenous and gradient-containing solutions; the last case we offer as approximation of the conditions, in which the nucleic acids exist in vivo.
    VL  - 3
    IS  - 1
    ER  - 

    Copy | Download

Author Information
  • Research and Training Center ‘Physical and Chemical Materials Science’ under Kyiv Taras Shevchenko University and NAS of Ukraine, Kiev, Ukraine

  • Sections