The original limited proteolysis approach was proposed for large (multidomain) proteins with post-translational modifications for obtaining of their novel fragments. It was realized for human immunoglobulins representing two subclasses IgG2 and IgG3. This approach was based on two techniques: masking of protein regions which are normally susceptible to proteolytic enzymes and increasing the possibility of proteolysis for sites which are not ordinarily accessible to these enzymes. The masking of immunoglobulin part which is sensitive to proteolysis was performed by Fab fragments (Fv subfragments) of antibodies and the increase of lability of stable regions was realized by pH change and mild reduction of disulfide bonds.
| Published in | International Journal of Immunology (Volume 2, Issue 3) |
| DOI | 10.11648/j.iji.20140203.11 |
| Page(s) | 16-23 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2014. Published by Science Publishing Group |
Limited Proteolysis, Immunoglobulin, Hinge, Fc Fragment, Facb Fragment
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APA Style
Maria Timchenko, Vladimir Tischenko. (2014). General Approach for Isolation of Immunoglobulins Fragments with the Core Hinge. International Journal of Immunology, 2(3), 16-23. https://doi.org/10.11648/j.iji.20140203.11
ACS Style
Maria Timchenko; Vladimir Tischenko. General Approach for Isolation of Immunoglobulins Fragments with the Core Hinge. Int. J. Immunol. 2014, 2(3), 16-23. doi: 10.11648/j.iji.20140203.11
AMA Style
Maria Timchenko, Vladimir Tischenko. General Approach for Isolation of Immunoglobulins Fragments with the Core Hinge. Int J Immunol. 2014;2(3):16-23. doi: 10.11648/j.iji.20140203.11
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Download@article{10.11648/j.iji.20140203.11,
author = {Maria Timchenko and Vladimir Tischenko},
title = {General Approach for Isolation of Immunoglobulins Fragments with the Core Hinge},
journal = {International Journal of Immunology},
volume = {2},
number = {3},
pages = {16-23},
doi = {10.11648/j.iji.20140203.11},
url = {https://doi.org/10.11648/j.iji.20140203.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20140203.11},
abstract = {The original limited proteolysis approach was proposed for large (multidomain) proteins with post-translational modifications for obtaining of their novel fragments. It was realized for human immunoglobulins representing two subclasses IgG2 and IgG3. This approach was based on two techniques: masking of protein regions which are normally susceptible to proteolytic enzymes and increasing the possibility of proteolysis for sites which are not ordinarily accessible to these enzymes. The masking of immunoglobulin part which is sensitive to proteolysis was performed by Fab fragments (Fv subfragments) of antibodies and the increase of lability of stable regions was realized by pH change and mild reduction of disulfide bonds.},
year = {2014}
}
TY - JOUR T1 - General Approach for Isolation of Immunoglobulins Fragments with the Core Hinge AU - Maria Timchenko AU - Vladimir Tischenko Y1 - 2014/11/18 PY - 2014 N1 - https://doi.org/10.11648/j.iji.20140203.11 DO - 10.11648/j.iji.20140203.11 T2 - International Journal of Immunology JF - International Journal of Immunology JO - International Journal of Immunology SP - 16 EP - 23 PB - Science Publishing Group SN - 2329-1753 UR - https://doi.org/10.11648/j.iji.20140203.11 AB - The original limited proteolysis approach was proposed for large (multidomain) proteins with post-translational modifications for obtaining of their novel fragments. It was realized for human immunoglobulins representing two subclasses IgG2 and IgG3. This approach was based on two techniques: masking of protein regions which are normally susceptible to proteolytic enzymes and increasing the possibility of proteolysis for sites which are not ordinarily accessible to these enzymes. The masking of immunoglobulin part which is sensitive to proteolysis was performed by Fab fragments (Fv subfragments) of antibodies and the increase of lability of stable regions was realized by pH change and mild reduction of disulfide bonds. VL - 2 IS - 3 ER -
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