Extracellular DNA-containing substances can be used as a marker of the balance between two processes: elimination of cells with critical number of lesions and repair of genetic material due to the work of adaptive systems. The idea of using this criterion was inspired by the results of measuring exDNA in blood plasma of chronically irradiated individuals. Here we used material obtained after gamma-irradiation (total dose 0.5 Gy) and subsequent culturing of PHA-stimulated human peripheral blood lymphocytes. The culture medium and exDNA isolated by various methods were treated with DNase and pronase. The concentration of free exDNA isolated by phenol extraction was an individual sign. The culture medium supernatant contained considerable amounts of exDNA in the form of nucleoprotein; cleavage of the protein component of this nucleoprotein considerably increased the size of fragments (from 1 to 20 kb) and reduced their resistance to DNase.
| Published in |
American Journal of Life Sciences (Volume 3, Issue 1-2)
This article belongs to the Special Issue Space Flight Factors: From Cell to Body |
| DOI | 10.11648/j.ajls.s.2015030102.11 |
| Page(s) | 1-4 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Extracellular DNA, Laser Correlation Spectroscopy, Radiation
| [1] | NN Veĭko, AV Ermakov, NA Egolina, NA Liapunova, DM Spitkovskiĭ, “Activation of total and ribosomal RNA transcription under adapting doses of ionizing radiation inducing displacement of chromosome loci in human G0-lymphocyte,” Radiats Biol Radioecol., vol. 44, No 5, 2004, pp.501-508. |
| [2] | AV Ermakov, MS Konkova, SV Kostyuk, VL Izevskaya, AV Baranova, NN Veiko, “Oxidized extracellular DNA as a stress signal in human cells,” Oxid Med Cell Longev. 2013;2013:649747. doi: 10.1155/2013/649747 |
| [3] | Klokov D., Criswell T., Leskov K.S., Araki S., Mayo L., Boothman D.A, “IR-inducible clusterin gene expression: a protein with potential roles in ionizing radiation-induced adaptive responses, genomic instability, and bystander effects,” Mutat. Res., vol. 568, No. 1, 2004, pp.97-110 |
| [4] | Vogelstein B. “Preparative and analytical purification of DNA from agarose,” Proc Natl Acad Sci U S A, vol. 76, No. 2, 1979, pp. 615-619 |
| [5] | Karganov M., Alchinova I., Arkhipova E., Skalny A.V., “Laser Correlation Spectroscopy: Nutritional, Ecological and Toxic Aspects,” In: “Biophysics”. A.N. Misra ed.- InTech, 2012, -ISBN 978-953-51-0376-9. P.1-16. DOI: 10.5772/35254 |
| [6] | Kostiuk SV, Zamulaeva IA, Agapova RK, Ermakov AV, Saenko AS, Orlova NV, Smirnova SG, Veĭko NN, Spitkovskiĭ DM., “The changing of cell-free DNA properties of peripheral blood and TCR-mutant cell frequency in individuals exposed to ionizing radiation,” Radiats Biol Radioecol, vol. 48, No.1, 2008, pp.5-13. |
| [7] | Alchinova I., Arkhipova E., Cherepov A., Karganov M., “Polysystemic monitoring of nuclear plant stuff and risk group revealing,” In: Risk assessment and management. Zhang Zhiyong ed., 2012, Academy Publish, USA. ISBN: 978-0-9835850-2-2. pp. 193-199. |
| [8] | Vral A., Thierens H., Baeyens A., De, Ridder L., “Chromosomal aberrations and in vitro radiosensitivity: intra-individual versus inter-individual variability,” Toxicol. Lett., vol. 149, 2004, pp. 345-352 |
| [9] | Anderson H.C., “The spontaneous frequency of chromosomal aberrations and sister-chromatid exchanges in cultured peripheral lymphocytes of a single blood donor sampled more than 200 times,” Mutat. Res., 1993, pp.281-292 |
| [10] | Kadhim M.A., Lee R., Moore S.R., Macdonald D.A., Chapman K.L., Patel G., Prise K.M., “Genomic instability after targeted irradiation of human lymphocytes: Evidence for inter-individual differences under bystander conditions,” Mutat. Res., vol. 688, No. 1-2, 2010, pp.91-94 |
| [11] | Ermakov AV, Konkova MS, Kostyuk SV, Smirnova TD, Malinovskaya EM, Efremova LV, Veiko NN., “An extracellular DNA mediated bystander effect produced from low dose irradiated endothelial cells,” Mutat Res, vol. 712(1-2), 2011, pp.1-10. doi: 10.1016/j.mrfmmm.2011.03.002 |
APA Style
Irina Alchinova. (2014). Changes in Serum and Cell Homeostasis under Irradiation: Factors of Distant Action. American Journal of Life Sciences, 3(1-2), 1-4. https://doi.org/10.11648/j.ajls.s.2015030102.11
ACS Style
Irina Alchinova. Changes in Serum and Cell Homeostasis under Irradiation: Factors of Distant Action. Am. J. Life Sci. 2014, 3(1-2), 1-4. doi: 10.11648/j.ajls.s.2015030102.11
AMA Style
Irina Alchinova. Changes in Serum and Cell Homeostasis under Irradiation: Factors of Distant Action. Am J Life Sci. 2014;3(1-2):1-4. doi: 10.11648/j.ajls.s.2015030102.11
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ajls.s.2015030102.11,
author = {Irina Alchinova},
title = {Changes in Serum and Cell Homeostasis under Irradiation: Factors of Distant Action},
journal = {American Journal of Life Sciences},
volume = {3},
number = {1-2},
pages = {1-4},
doi = {10.11648/j.ajls.s.2015030102.11},
url = {https://doi.org/10.11648/j.ajls.s.2015030102.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2015030102.11},
abstract = {Extracellular DNA-containing substances can be used as a marker of the balance between two processes: elimination of cells with critical number of lesions and repair of genetic material due to the work of adaptive systems. The idea of using this criterion was inspired by the results of measuring exDNA in blood plasma of chronically irradiated individuals. Here we used material obtained after gamma-irradiation (total dose 0.5 Gy) and subsequent culturing of PHA-stimulated human peripheral blood lymphocytes. The culture medium and exDNA isolated by various methods were treated with DNase and pronase. The concentration of free exDNA isolated by phenol extraction was an individual sign. The culture medium supernatant contained considerable amounts of exDNA in the form of nucleoprotein; cleavage of the protein component of this nucleoprotein considerably increased the size of fragments (from 1 to 20 kb) and reduced their resistance to DNase.},
year = {2014}
}
TY - JOUR T1 - Changes in Serum and Cell Homeostasis under Irradiation: Factors of Distant Action AU - Irina Alchinova Y1 - 2014/10/25 PY - 2014 N1 - https://doi.org/10.11648/j.ajls.s.2015030102.11 DO - 10.11648/j.ajls.s.2015030102.11 T2 - American Journal of Life Sciences JF - American Journal of Life Sciences JO - American Journal of Life Sciences SP - 1 EP - 4 PB - Science Publishing Group SN - 2328-5737 UR - https://doi.org/10.11648/j.ajls.s.2015030102.11 AB - Extracellular DNA-containing substances can be used as a marker of the balance between two processes: elimination of cells with critical number of lesions and repair of genetic material due to the work of adaptive systems. The idea of using this criterion was inspired by the results of measuring exDNA in blood plasma of chronically irradiated individuals. Here we used material obtained after gamma-irradiation (total dose 0.5 Gy) and subsequent culturing of PHA-stimulated human peripheral blood lymphocytes. The culture medium and exDNA isolated by various methods were treated with DNase and pronase. The concentration of free exDNA isolated by phenol extraction was an individual sign. The culture medium supernatant contained considerable amounts of exDNA in the form of nucleoprotein; cleavage of the protein component of this nucleoprotein considerably increased the size of fragments (from 1 to 20 kb) and reduced their resistance to DNase. VL - 3 IS - 1-2 ER -
Email: