Isolation, Cloning and Expression of Rhamnolipid Operon from <i>Pseudomonas aeroginosa</i> ATCC 9027 in Logarithmic Phase in <i>E. coli</i> BL21

Amin Jafari; Jamshid Raheb; Hassan Bardania; Behnam Rasekh

doi:doi:10.11648/j.ajls.s.2014020603.15

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Isolation, Cloning and Expression of Rhamnolipid Operon from Pseudomonas aeroginosa ATCC 9027 in Logarithmic Phase in E. coli BL21

Amin Jafari, Jamshid Raheb, Hassan Bardania, Behnam Rasekh

Published in American Journal of Life Sciences (Volume 2, Issue 6-3)

Received: 19 October 2014 Accepted: 8 December 2014 Published: 23 March 2017

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Abstract

The rhamnolipid operon from Pesudomonas strain with the native promoter was not expressed in logarithmic phase of Ecoli. The expression of rhamnolipid in logarithmic phase of growth whether the regulatory elements of the operon are eliminated or not was investigated. The rhamnolipid operon was identified in Pseudomonas aeruginosa ATCC 9027 and the rhlAB genes related to rhamnolipid were isolated and amplified by PCR. The PCR product was cloned in pET 23a expression vector and transferred into the E. coli BL21. The expression of rhlAB genes was analyzed and our results showed that the synthesis of monorhamnolipid occurred in logarithmic phase. In addition this data demonstrated a higher production of rhamnolipid in recombinant Ecoli Bl21compared to that indigenous Pseudomonas aeruginosa ATCC 9027.

Published in	American Journal of Life Sciences (Volume 2, Issue 6-3) This article belongs to the Special Issue Microbiology Research
DOI	10.11648/j.ajls.s.2014020603.15
Page(s)	22-30
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

MEOR, Manipulated E. coli BL21, Monorhamnolipid, Logarithmic Phase

References

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APA Style

Amin Jafari, Jamshid Raheb, Hassan Bardania, Behnam Rasekh. (2017). Isolation, Cloning and Expression of Rhamnolipid Operon from Pseudomonas aeroginosa ATCC 9027 in Logarithmic Phase in E. coli BL21. American Journal of Life Sciences, 2(6-3), 22-30. https://doi.org/10.11648/j.ajls.s.2014020603.15

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ACS Style

Amin Jafari; Jamshid Raheb; Hassan Bardania; Behnam Rasekh. Isolation, Cloning and Expression of Rhamnolipid Operon from Pseudomonas aeroginosa ATCC 9027 in Logarithmic Phase in E. coli BL21. Am. J. Life Sci. 2017, 2(6-3), 22-30. doi: 10.11648/j.ajls.s.2014020603.15

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AMA Style

Amin Jafari, Jamshid Raheb, Hassan Bardania, Behnam Rasekh. Isolation, Cloning and Expression of Rhamnolipid Operon from Pseudomonas aeroginosa ATCC 9027 in Logarithmic Phase in E. coli BL21. Am J Life Sci. 2017;2(6-3):22-30. doi: 10.11648/j.ajls.s.2014020603.15

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@article{10.11648/j.ajls.s.2014020603.15,
  author = {Amin Jafari and Jamshid Raheb and Hassan Bardania and Behnam Rasekh},
  title = {Isolation, Cloning and Expression of Rhamnolipid Operon from Pseudomonas aeroginosa ATCC 9027 in Logarithmic Phase in E. coli BL21},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {6-3},
  pages = {22-30},
  doi = {10.11648/j.ajls.s.2014020603.15},
  url = {https://doi.org/10.11648/j.ajls.s.2014020603.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2014020603.15},
  abstract = {The rhamnolipid operon from Pesudomonas strain with the native promoter was not expressed in logarithmic phase of Ecoli. The expression of rhamnolipid in logarithmic phase of growth whether the regulatory elements of the operon are eliminated or not was investigated. The rhamnolipid operon was identified in Pseudomonas aeruginosa ATCC 9027 and the rhlAB genes related to rhamnolipid were isolated and amplified by PCR. The PCR product was cloned in pET 23a expression vector and transferred into the E. coli BL21. The expression of rhlAB genes was analyzed and our results showed that the synthesis of monorhamnolipid occurred in logarithmic phase. In addition this data demonstrated a higher production of rhamnolipid in recombinant Ecoli Bl21compared to that indigenous Pseudomonas aeruginosa ATCC 9027.},
 year = {2017}
}

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TY  - JOUR
T1  - Isolation, Cloning and Expression of Rhamnolipid Operon from Pseudomonas aeroginosa ATCC 9027 in Logarithmic Phase in E. coli BL21
AU  - Amin Jafari
AU  - Jamshid Raheb
AU  - Hassan Bardania
AU  - Behnam Rasekh
Y1  - 2017/03/23
PY  - 2017
N1  - https://doi.org/10.11648/j.ajls.s.2014020603.15
DO  - 10.11648/j.ajls.s.2014020603.15
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 22
EP  - 30
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.s.2014020603.15
AB  - The rhamnolipid operon from Pesudomonas strain with the native promoter was not expressed in logarithmic phase of Ecoli. The expression of rhamnolipid in logarithmic phase of growth whether the regulatory elements of the operon are eliminated or not was investigated. The rhamnolipid operon was identified in Pseudomonas aeruginosa ATCC 9027 and the rhlAB genes related to rhamnolipid were isolated and amplified by PCR. The PCR product was cloned in pET 23a expression vector and transferred into the E. coli BL21. The expression of rhlAB genes was analyzed and our results showed that the synthesis of monorhamnolipid occurred in logarithmic phase. In addition this data demonstrated a higher production of rhamnolipid in recombinant Ecoli Bl21compared to that indigenous Pseudomonas aeruginosa ATCC 9027.
VL  - 2
IS  - 6-3
ER  -

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Author Information

Amin Jafari

National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Jamshid Raheb

National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Hassan Bardania

National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Behnam Rasekh

Department of Petroleum Biotechnology, Biotechnology Research Center, Research Institute of Petroleum Industry, Tehran, Iran

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Plain Text BibTeX RIS

APA Style

Amin Jafari, Jamshid Raheb, Hassan Bardania, Behnam Rasekh. (2017). Isolation, Cloning and Expression of Rhamnolipid Operon from Pseudomonas aeroginosa ATCC 9027 in Logarithmic Phase in E. coli BL21. American Journal of Life Sciences, 2(6-3), 22-30. https://doi.org/10.11648/j.ajls.s.2014020603.15

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ACS Style

Amin Jafari; Jamshid Raheb; Hassan Bardania; Behnam Rasekh. Isolation, Cloning and Expression of Rhamnolipid Operon from Pseudomonas aeroginosa ATCC 9027 in Logarithmic Phase in E. coli BL21. Am. J. Life Sci. 2017, 2(6-3), 22-30. doi: 10.11648/j.ajls.s.2014020603.15

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AMA Style

Amin Jafari, Jamshid Raheb, Hassan Bardania, Behnam Rasekh. Isolation, Cloning and Expression of Rhamnolipid Operon from Pseudomonas aeroginosa ATCC 9027 in Logarithmic Phase in E. coli BL21. Am J Life Sci. 2017;2(6-3):22-30. doi: 10.11648/j.ajls.s.2014020603.15

Copy | Download

@article{10.11648/j.ajls.s.2014020603.15,
  author = {Amin Jafari and Jamshid Raheb and Hassan Bardania and Behnam Rasekh},
  title = {Isolation, Cloning and Expression of Rhamnolipid Operon from Pseudomonas aeroginosa ATCC 9027 in Logarithmic Phase in E. coli BL21},
  journal = {American Journal of Life Sciences},
  volume = {2},
  number = {6-3},
  pages = {22-30},
  doi = {10.11648/j.ajls.s.2014020603.15},
  url = {https://doi.org/10.11648/j.ajls.s.2014020603.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajls.s.2014020603.15},
  abstract = {The rhamnolipid operon from Pesudomonas strain with the native promoter was not expressed in logarithmic phase of Ecoli. The expression of rhamnolipid in logarithmic phase of growth whether the regulatory elements of the operon are eliminated or not was investigated. The rhamnolipid operon was identified in Pseudomonas aeruginosa ATCC 9027 and the rhlAB genes related to rhamnolipid were isolated and amplified by PCR. The PCR product was cloned in pET 23a expression vector and transferred into the E. coli BL21. The expression of rhlAB genes was analyzed and our results showed that the synthesis of monorhamnolipid occurred in logarithmic phase. In addition this data demonstrated a higher production of rhamnolipid in recombinant Ecoli Bl21compared to that indigenous Pseudomonas aeruginosa ATCC 9027.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Isolation, Cloning and Expression of Rhamnolipid Operon from Pseudomonas aeroginosa ATCC 9027 in Logarithmic Phase in E. coli BL21
AU  - Amin Jafari
AU  - Jamshid Raheb
AU  - Hassan Bardania
AU  - Behnam Rasekh
Y1  - 2017/03/23
PY  - 2017
N1  - https://doi.org/10.11648/j.ajls.s.2014020603.15
DO  - 10.11648/j.ajls.s.2014020603.15
T2  - American Journal of Life Sciences
JF  - American Journal of Life Sciences
JO  - American Journal of Life Sciences
SP  - 22
EP  - 30
PB  - Science Publishing Group
SN  - 2328-5737
UR  - https://doi.org/10.11648/j.ajls.s.2014020603.15
AB  - The rhamnolipid operon from Pesudomonas strain with the native promoter was not expressed in logarithmic phase of Ecoli. The expression of rhamnolipid in logarithmic phase of growth whether the regulatory elements of the operon are eliminated or not was investigated. The rhamnolipid operon was identified in Pseudomonas aeruginosa ATCC 9027 and the rhlAB genes related to rhamnolipid were isolated and amplified by PCR. The PCR product was cloned in pET 23a expression vector and transferred into the E. coli BL21. The expression of rhlAB genes was analyzed and our results showed that the synthesis of monorhamnolipid occurred in logarithmic phase. In addition this data demonstrated a higher production of rhamnolipid in recombinant Ecoli Bl21compared to that indigenous Pseudomonas aeruginosa ATCC 9027.
VL  - 2
IS  - 6-3
ER  -

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