Introduction: Dysfunction, degeneration, and loss of retinal pigment epithelial (RPE) cells are prominent features of subtypes of retinitis pigmentosa (RP) which is a cause of visual disability. In this condition, there is progressive visual loss that often leads to blindness. A variety of therapeutic approaches to delay the degenerative process are under development, the most promising of which is the stem cell regenerative therapy. Stem cells are self-renewing cells capable of transdifferentiation into a number of cell lineages. Mesenchymal stem cells (MSCs) represent a type of stem cell with remarkable proliferative, regenerative and transdifferentiation ability. Aim of the Work: The aim of this work was to evaluate the ability of bone marrow derived MSCs to differentiate into retinal pigmented epithelial cells in vitro as a step towards regeneration of degenerated retinal cells as in cases of retinitis pigmentosa. Patients and Methods: The present study was carried out on 20 bone marrow samples obtained from donors undergoing bone marrow aspirations, at EL-Maadi Military Hospital. 3 ml bone marrow were aspirated, mesenchymal stem cells were separated and induced to RPE lineage through culture in the presence of nicotinamide NIC and factors from the TGF-b superfamily (Activin A). The BMSC- derived pigmented-like cells were evaluated for the morphology, function, and marker expression of authentic RPE using RT-PCR for RPE65 mRNA. Results: Bone marrow derived mesenchymal stem cells in all samples demonstrated the morphologic characteristics as well as molecular markers of RPE cells after 8 weeks of culture with activin A and nicotinamide. Conclusion: In the present study, compelling evidences support the possibility of differentiation of bone marrow stem cells into retinal pigmented epithelial cells (RPE) which have the morphology, function, and marker expression of authentic RPE.
| Published in |
American Journal of Bioscience and Bioengineering (Volume 3, Issue 4-1)
This article belongs to the Special Issue Stem Cells for Neuro-Regeneration: Where Do We Stand |
| DOI | 10.11648/j.bio.s.2015030401.18 |
| Page(s) | 51-55 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Retinal Pigment Epithelial (RPE), Retinitis Pigmentosa (RP), Nicotinamide (NIC), Activin A, Bone Marrow Derived Stem Cells (BMSC)
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APA Style
Nehal Draz, Makram F. Attalah, Hany M. Shaheen, Wael Abo Elkeir. (2015). In Vitro Differentiation of Human Bone Marrow Stem Cells into Retinal Pigment Epithelium. American Journal of Bioscience and Bioengineering, 3(4-1), 51-55. https://doi.org/10.11648/j.bio.s.2015030401.18
ACS Style
Nehal Draz; Makram F. Attalah; Hany M. Shaheen; Wael Abo Elkeir. In Vitro Differentiation of Human Bone Marrow Stem Cells into Retinal Pigment Epithelium. Am. J. BioSci. Bioeng. 2015, 3(4-1), 51-55. doi: 10.11648/j.bio.s.2015030401.18
AMA Style
Nehal Draz, Makram F. Attalah, Hany M. Shaheen, Wael Abo Elkeir. In Vitro Differentiation of Human Bone Marrow Stem Cells into Retinal Pigment Epithelium. Am J BioSci Bioeng. 2015;3(4-1):51-55. doi: 10.11648/j.bio.s.2015030401.18
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Download@article{10.11648/j.bio.s.2015030401.18,
author = {Nehal Draz and Makram F. Attalah and Hany M. Shaheen and Wael Abo Elkeir},
title = {In Vitro Differentiation of Human Bone Marrow Stem Cells into Retinal Pigment Epithelium},
journal = {American Journal of Bioscience and Bioengineering},
volume = {3},
number = {4-1},
pages = {51-55},
doi = {10.11648/j.bio.s.2015030401.18},
url = {https://doi.org/10.11648/j.bio.s.2015030401.18},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.s.2015030401.18},
abstract = {Introduction: Dysfunction, degeneration, and loss of retinal pigment epithelial (RPE) cells are prominent features of subtypes of retinitis pigmentosa (RP) which is a cause of visual disability. In this condition, there is progressive visual loss that often leads to blindness. A variety of therapeutic approaches to delay the degenerative process are under development, the most promising of which is the stem cell regenerative therapy. Stem cells are self-renewing cells capable of transdifferentiation into a number of cell lineages. Mesenchymal stem cells (MSCs) represent a type of stem cell with remarkable proliferative, regenerative and transdifferentiation ability. Aim of the Work: The aim of this work was to evaluate the ability of bone marrow derived MSCs to differentiate into retinal pigmented epithelial cells in vitro as a step towards regeneration of degenerated retinal cells as in cases of retinitis pigmentosa. Patients and Methods: The present study was carried out on 20 bone marrow samples obtained from donors undergoing bone marrow aspirations, at EL-Maadi Military Hospital. 3 ml bone marrow were aspirated, mesenchymal stem cells were separated and induced to RPE lineage through culture in the presence of nicotinamide NIC and factors from the TGF-b superfamily (Activin A). The BMSC- derived pigmented-like cells were evaluated for the morphology, function, and marker expression of authentic RPE using RT-PCR for RPE65 mRNA. Results: Bone marrow derived mesenchymal stem cells in all samples demonstrated the morphologic characteristics as well as molecular markers of RPE cells after 8 weeks of culture with activin A and nicotinamide. Conclusion: In the present study, compelling evidences support the possibility of differentiation of bone marrow stem cells into retinal pigmented epithelial cells (RPE) which have the morphology, function, and marker expression of authentic RPE.},
year = {2015}
}
TY - JOUR T1 - In Vitro Differentiation of Human Bone Marrow Stem Cells into Retinal Pigment Epithelium AU - Nehal Draz AU - Makram F. Attalah AU - Hany M. Shaheen AU - Wael Abo Elkeir Y1 - 2015/07/06 PY - 2015 N1 - https://doi.org/10.11648/j.bio.s.2015030401.18 DO - 10.11648/j.bio.s.2015030401.18 T2 - American Journal of Bioscience and Bioengineering JF - American Journal of Bioscience and Bioengineering JO - American Journal of Bioscience and Bioengineering SP - 51 EP - 55 PB - Science Publishing Group SN - 2328-5893 UR - https://doi.org/10.11648/j.bio.s.2015030401.18 AB - Introduction: Dysfunction, degeneration, and loss of retinal pigment epithelial (RPE) cells are prominent features of subtypes of retinitis pigmentosa (RP) which is a cause of visual disability. In this condition, there is progressive visual loss that often leads to blindness. A variety of therapeutic approaches to delay the degenerative process are under development, the most promising of which is the stem cell regenerative therapy. Stem cells are self-renewing cells capable of transdifferentiation into a number of cell lineages. Mesenchymal stem cells (MSCs) represent a type of stem cell with remarkable proliferative, regenerative and transdifferentiation ability. Aim of the Work: The aim of this work was to evaluate the ability of bone marrow derived MSCs to differentiate into retinal pigmented epithelial cells in vitro as a step towards regeneration of degenerated retinal cells as in cases of retinitis pigmentosa. Patients and Methods: The present study was carried out on 20 bone marrow samples obtained from donors undergoing bone marrow aspirations, at EL-Maadi Military Hospital. 3 ml bone marrow were aspirated, mesenchymal stem cells were separated and induced to RPE lineage through culture in the presence of nicotinamide NIC and factors from the TGF-b superfamily (Activin A). The BMSC- derived pigmented-like cells were evaluated for the morphology, function, and marker expression of authentic RPE using RT-PCR for RPE65 mRNA. Results: Bone marrow derived mesenchymal stem cells in all samples demonstrated the morphologic characteristics as well as molecular markers of RPE cells after 8 weeks of culture with activin A and nicotinamide. Conclusion: In the present study, compelling evidences support the possibility of differentiation of bone marrow stem cells into retinal pigmented epithelial cells (RPE) which have the morphology, function, and marker expression of authentic RPE. VL - 3 IS - 4-1 ER -
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