Background: Mesenchymal stem cells (MSCs) are multipotent adult stem cells present in all tissues. They are present in bone marrow, and can differentiate in vitro into neurons, glial cells and myofibroblasts . MSCs have been proposed as sources of stem cells for regeneration of the CNS. Thus, one of the goals of regenerative medicine is to regenerate damaged brain tissue and spinal cord by harnessing the power of stem cells to initiate neurogenesis in damaged areas of the brain. Objective: The aim of this work is to study in-vitro induced neurogenesis using MSCs as model of stem cells. Methodology : Bone marrow-MSCs were isolated, expanded and passaged. MSCs were identified using morphology and flowcytometric analysis. Co-expression of Oct ¾ was done. MSCs were induced to neural lineage using Neural Induction Media (NIM) : a cocktail of retinoic acid dissolved in DEMSO, recombinant human Fibroblast Growth Factor (FGF) basic, recombinant human Epidermal Growth Factor (EGF) and Insulin-like Growth Factor I (IGF-I) . Neural induction was verified morphologically, and immunologically using GFAP positivity and nestin expression. Results: BM-MSCs express CD44 and OCT ¾ which decrease with age. MSCs induced with NIM show morphological changes consistent with neurogenesis, positive GFAP and nestin expression as compared to the uninduced cells. Conclusion: MSCs isolated from bone marrow aspirate and can be differentiated into GFAP positive neural cells.
| Published in |
American Journal of Bioscience and Bioengineering (Volume 3, Issue 4-1)
This article belongs to the Special Issue Stem Cells for Neuro-Regeneration: Where Do We Stand |
| DOI | 10.11648/j.bio.s.2015030401.17 |
| Page(s) | 43-50 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Mesenchymal Stem Cells, GFAP, Retinoic Acid, Neurogenesis
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APA Style
Ahmedy E., Kandel S., Gabr H., Rizk S., Khalifa . K, et al. (2015). Neurogenic Differentiation of Bone Marrow-derived Mesenchymal Stem Cells Using Neural Induction Medium: A Morphological and Histochemical Study. American Journal of Bioscience and Bioengineering, 3(4-1), 43-50. https://doi.org/10.11648/j.bio.s.2015030401.17
ACS Style
Ahmedy E.; Kandel S.; Gabr H.; Rizk S.; Khalifa . K, et al. Neurogenic Differentiation of Bone Marrow-derived Mesenchymal Stem Cells Using Neural Induction Medium: A Morphological and Histochemical Study. Am. J. BioSci. Bioeng. 2015, 3(4-1), 43-50. doi: 10.11648/j.bio.s.2015030401.17
AMA Style
Ahmedy E., Kandel S., Gabr H., Rizk S., Khalifa . K, et al. Neurogenic Differentiation of Bone Marrow-derived Mesenchymal Stem Cells Using Neural Induction Medium: A Morphological and Histochemical Study. Am J BioSci Bioeng. 2015;3(4-1):43-50. doi: 10.11648/j.bio.s.2015030401.17
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Download@article{10.11648/j.bio.s.2015030401.17,
author = {Ahmedy E. and Kandel S. and Gabr H. and Rizk S. and Khalifa . K and Kamal S.},
title = {Neurogenic Differentiation of Bone Marrow-derived Mesenchymal Stem Cells Using Neural Induction Medium: A Morphological and Histochemical Study},
journal = {American Journal of Bioscience and Bioengineering},
volume = {3},
number = {4-1},
pages = {43-50},
doi = {10.11648/j.bio.s.2015030401.17},
url = {https://doi.org/10.11648/j.bio.s.2015030401.17},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.s.2015030401.17},
abstract = {Background: Mesenchymal stem cells (MSCs) are multipotent adult stem cells present in all tissues. They are present in bone marrow, and can differentiate in vitro into neurons, glial cells and myofibroblasts . MSCs have been proposed as sources of stem cells for regeneration of the CNS. Thus, one of the goals of regenerative medicine is to regenerate damaged brain tissue and spinal cord by harnessing the power of stem cells to initiate neurogenesis in damaged areas of the brain. Objective: The aim of this work is to study in-vitro induced neurogenesis using MSCs as model of stem cells. Methodology : Bone marrow-MSCs were isolated, expanded and passaged. MSCs were identified using morphology and flowcytometric analysis. Co-expression of Oct ¾ was done. MSCs were induced to neural lineage using Neural Induction Media (NIM) : a cocktail of retinoic acid dissolved in DEMSO, recombinant human Fibroblast Growth Factor (FGF) basic, recombinant human Epidermal Growth Factor (EGF) and Insulin-like Growth Factor I (IGF-I) . Neural induction was verified morphologically, and immunologically using GFAP positivity and nestin expression. Results: BM-MSCs express CD44 and OCT ¾ which decrease with age. MSCs induced with NIM show morphological changes consistent with neurogenesis, positive GFAP and nestin expression as compared to the uninduced cells. Conclusion: MSCs isolated from bone marrow aspirate and can be differentiated into GFAP positive neural cells.},
year = {2015}
}
TY - JOUR T1 - Neurogenic Differentiation of Bone Marrow-derived Mesenchymal Stem Cells Using Neural Induction Medium: A Morphological and Histochemical Study AU - Ahmedy E. AU - Kandel S. AU - Gabr H. AU - Rizk S. AU - Khalifa . K AU - Kamal S. Y1 - 2015/07/06 PY - 2015 N1 - https://doi.org/10.11648/j.bio.s.2015030401.17 DO - 10.11648/j.bio.s.2015030401.17 T2 - American Journal of Bioscience and Bioengineering JF - American Journal of Bioscience and Bioengineering JO - American Journal of Bioscience and Bioengineering SP - 43 EP - 50 PB - Science Publishing Group SN - 2328-5893 UR - https://doi.org/10.11648/j.bio.s.2015030401.17 AB - Background: Mesenchymal stem cells (MSCs) are multipotent adult stem cells present in all tissues. They are present in bone marrow, and can differentiate in vitro into neurons, glial cells and myofibroblasts . MSCs have been proposed as sources of stem cells for regeneration of the CNS. Thus, one of the goals of regenerative medicine is to regenerate damaged brain tissue and spinal cord by harnessing the power of stem cells to initiate neurogenesis in damaged areas of the brain. Objective: The aim of this work is to study in-vitro induced neurogenesis using MSCs as model of stem cells. Methodology : Bone marrow-MSCs were isolated, expanded and passaged. MSCs were identified using morphology and flowcytometric analysis. Co-expression of Oct ¾ was done. MSCs were induced to neural lineage using Neural Induction Media (NIM) : a cocktail of retinoic acid dissolved in DEMSO, recombinant human Fibroblast Growth Factor (FGF) basic, recombinant human Epidermal Growth Factor (EGF) and Insulin-like Growth Factor I (IGF-I) . Neural induction was verified morphologically, and immunologically using GFAP positivity and nestin expression. Results: BM-MSCs express CD44 and OCT ¾ which decrease with age. MSCs induced with NIM show morphological changes consistent with neurogenesis, positive GFAP and nestin expression as compared to the uninduced cells. Conclusion: MSCs isolated from bone marrow aspirate and can be differentiated into GFAP positive neural cells. VL - 3 IS - 4-1 ER -
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